Journal: Cells

Article Title: Effect of Long-Term 3D Spheroid Culture on WJ-MSC

doi: 10.3390/cells10040719

Figure Lengend Snippet: List of primary antibodies used for immunocytochemistry.

Article Snippet: Nestin , Mouse monoclonal , IgG1 , 1:500 , Merck Millipore , MAB5326.

Techniques: Immunocytochemistry

Journal: PLoS ONE

Article Title: Development of novel monoclonal antibodies with specific binding affinity for denatured human CD26 in formalin-fixed paraffin-embedded and decalcified specimens

doi: 10.1371/journal.pone.0218330

Figure Lengend Snippet: MSTO parent, MSTO-CD26, JMN CD26-shRNA, JMN ctrl-shRNA, A549, Jurkat parent or Jurkat-CD26 cells were incubated with unlabeled isotype control or purified mouse anti-human CD26 mAb (19–32, U16-3 or U38-8) or purified goat anti-human CD26 pAb (R&D Systems), and subsequently stained with PE-labeled goat anti-mouse Ig pAb or PE-labeled donkey anti-goat IgG Ab, and analyzed by flow cytometry. PE-labeled commercial mouse anti-human CD26 mAb (BD Biosciences, clone M-A261) was utilized as a positive control. Representative data of three independent experiments are shown as mean ± S.D. of the mean fluorescence intensity (MFI) from triplicate samples, and similar results were obtained in each experiment.

Article Snippet: In brief, The cultured cell lines were incubated with 10 μg/ml of purified mouse anti-human CD26 mAb (19–32, U16-3 or U38-8) or goat anti-human CD26 pAb or isotype controls for 25 min at 4°C, and subsequently stained with PE-conjugated goat anti-mouse Ig pAb (BD Biosciences, San Jose, CA) or PE-conjugated donkey anti-goat IgG Ab (Santa Cruz Biotechnology) for 25 min at 4°C.

Techniques: shRNA, Incubation, Purification, Staining, Labeling, Flow Cytometry, Positive Control, Fluorescence